conclusions in this study, we isolated b. licheniformis producing an organic solvent-stable protease from oil-contaminated soil. the protease was stable and active in various organic solvents. by purification, the protease could be used as a biocatalyst for organic solvent-based enzymatic synthesis. results according to 16srdna sequencing, strain isa9 was identified as a new strain of bacillus ...

objective: investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (smf). methods: optimum temperature for enzyme activity in the crude extract was 40 ◦c at a ph between 8.0 and 9.0. the studies on ph stability showed that the enzyme was stab...

Penicilliurn expamum grown in a medium with rice husk as a carbon source produced an extracellular protease. The protease enzyme was isolated from culture broth by fractionation with acetone and column chromatography on Sephadex G- 100 and DEAE A-50. The protease enzyme was purified about 17.47 fold, with a recovery of 14%. The purified protease was homogenous on SDS polyacrylarnide disc ge...

the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...

Proteases are important industrial enzymes used in different areas of industry, mainly detergent, food and leather industries. In this study, novel alkaline protease-producing bacterium was isolated from Geinarje hot spring and examined for maximum protease activity to be utilized in washing-powder. The isolated bacterium was cultured in mineral salt medium including 2% Skim Milk. Proteolytic a...

proteases are important enzymes that their role in various industries is undeniable. however, keeping enzymes stable during its activity in harsh conditions is so important. in this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and phs was evaluated. first silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

hydatidosis is a zoonosis disease caused by the larva of echinococcus granulosus parasite in man and animals. the proteases enzymes are necessary for nutrition, migration and evasion of immunity of parasite into host. the aim of this study was to determine the protease activity of hydatid cyst protoscolices (psc), healthy and cystic liver tissues in order to compare of this biomarker for host a...

kinetics of bacterial growth and protease production were monitored on a novel isolated moderately halophilic bacterium, salinivibrio sp. strain ms-7, and maximum growth and protease activity was achieved after 48 hours at 30°c and 180 rpm. to determine the effect of various carbon sources on protease production, glucose, lactose, sucrose and maltose were investigated and  maximum production of...

The effect of different thiourea (TU) concentrations (10 and 20 mM) on antioxidants, protease activity and protein content with and without H2O2 (50 mM) induced stress was investigated in wheat leaves. A gradual decrease in protein content with a peak at 72 hours was observed in stressed as well as control leaves. This gradual decrease in leaf protein content at different time intervals was com...

Proteases are important enzymes that their role in various industries is undeniable. However, keeping enzymes stable during its activity in harsh conditions is so important. In this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and pHs was evaluated. First silica particles were aminated by 3-aminopropyltriethoxysilane then the ...